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To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis

Authors:

MS ANJUM ,

Assistant Consultant of Medicine King Abdullah Medical City, Makkah, Saudi Arabia., SA
About MS
Assistant Consultant of Medicine
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U ANSARI,

M.B.B.S, FCPS (Haematology) Assistant Professor of Pathology (Haematology) Independent Medical College, Faisalabad,, PK
About U
Assistant Professor of Pathology (Haematology)
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F HUSSAIN,

M.B.B.S, FCPS (Anaesthesiology) Assistant Professor, PK
About F
Combined Military Hospital, Rawalpindi, Pakistan.
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N KALSOOM

M.B.B.S Medical Officer National Hospital, Faisalabad, PK
About N

Medical Officer

National Hospital, Faisalabad, Pakistan.

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Abstract

Introduction: Leishmaniasis is a disease caused by a protozoan parasite of the genus Leishmania, which is transmitted through bites of infected sandflies. It has been reported that the polymerase chain reaction (PCR) is a more sensitive and specific test for the diagnosis of visceral leishmaniasis than bone marrow examination. This recent study is a renewed effort to validate the role of PCR in the diagnosis of visceral leishmaniasis.

Objective: The objective of this study was to determine the sensitivity and specificity of PCR in the diagnosis of visceral leishmaniasis.

Study design: Cross sectional (validation) study carried out in the Haematology department, Armed Forces Institute of Pathology, Rawalpindi. from 25th March 2011 to 24th March 2012.

Subjects and Methods: A total number of 59 patients with visceral leishmaniasis diagnosed on microscopic bone marrow examination with equal number of negative controls were studied. The subjects were tested for the presence of visceral leishmaniasis by the polymerase chain reaction.

Results: All the 59 patients were found to be positive for visceral leishmaniasis by PCR. None of the negative controls were positive by PCR.

Conclusion: The study validates that PCR is equal to microscopic bone marrow examination in the diagnosis of visceral leishmaniasis.

 

DOI: http://dx.doi.org/10.4038/sljid.v4i1.6036

How to Cite: ANJUM, M. et al., (2014). To determine the sensitivity and specificity of Polymerase chain reaction (PCR) in the diagnosis of visceral leishmaniasis. Sri Lankan Journal of Infectious Diseases. 4(1), pp.22–29. DOI: http://doi.org/10.4038/sljid.v4i1.6036
Published on 28 Feb 2014.
Peer Reviewed

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