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An audit on the technique of collection of blood for culture at the National Hospital of Sri Lanka

Authors:

Enoka Corea ,

Dept of Microbiology, Faculty of Medicine, University of Colombo, LK
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Nasra Ameen,

Dept of Microbiology, Faculty of Medicine, University of Colombo, LK
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Sanath Rajakaruna,

Dept of Microbiology, Faculty of Medicine, University of Colombo, LK
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Philomina Chandrasiri

National Hospital of Sri Lanka, LK
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Abstract

The accuracy of blood culture results is highly dependent on proper technique. This audit sought to determine whether health care personnel are adhering to standard techniques for collection of blood for culture. Fifty episodes of blood culture collection were observed at the National Hospital of Sri Lanka between August and December 2008 using an observational check list to determine accuracy of technique. 44/50 of the cultures were drawn after commencement of antibiotics. 45/50 did not wash their hands prior to collection. Of the five who washed their hands only two used antiseptic soap. 37/50 wore sterile gloves. 16/50 used two antiseptics and 32/50 used one to disinfect the venepuncture site. 2 did not disinfect the entry site. Only 2 swabbed the site in a concentric manner. 11 touched the site after disinfection. Only 39 were able to collect blood with a single puncture. Almost all the participants (49/50) did not disinfect the lid of the culture bottle. Only 21/50 drew adequate blood for blood culture. Only two drew a second blood culture. Syringes used to collect blood should be discarded into a sharp bin without recapping. In our study 40 /50 participants recapped the syringe before disposal. However 45 /50 of the participants correctly disposed the sharps and gloves into the sharp bin and yellow bag respectively. Although written instructions have been given regarding collection of blood for culture a high percentage of health care workers do not adhere to these guidelines. This is an important quality assurance issue that needs to be addressed.

DOI: http://dx.doi.org/10.4038/sljid.v1i1.3184

Sri Lankan Journal of Infectious Diseases Vol.1(1) 2011: 18-23

Keywords: Blood culture Quality 
How to Cite: Corea, E. et al., (2011). An audit on the technique of collection of blood for culture at the National Hospital of Sri Lanka. Sri Lankan Journal of Infectious Diseases. 1(1), pp.18–23. DOI: http://doi.org/10.4038/sljid.v1i1.3184
Published on 28 Jul 2011.
Peer Reviewed

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