Short Reports
Comparison of direct fluorescent test with real-time PCR to detect acute lower respiratory tract virus infections in children from May to July 2014, in a tertiary care hospital in Sri Lanka
Authors:
H. B. C. Harshani ,
Medical Research Institute, Colombo, LK
About H. B. C.
National Influenza Centre, Department of Virology
N. R. Ratnayake,
Lady Ridgeway Hospital, Colombo, LK
C. J. S. Jayamaha
Medical Research Institute, Colombo, LK
About C. J. S.
National Influenza Centre, Department of Virology
Abstract
In this study, direct fluorescent test (DFT) and real-time PCR (rtPCR) were compared for the detection of acute lower respiratory tract virus infections caused by respiratory syncytial virus (RSV) A and B, influenza (Flu) A and B, parainfluenza virus (PIV) 1, 2, 3 and 4 and adenovirus (AdV) in children admitted to the Lady Ridgeway Hospital, Colombo.
Nasopharyngeal aspirates/swabs were obtained from May to July, 2014. Cell pellets were subjected to DFT. Extracted viral RNA were subjected to four different real-time multiplex PCR assays to detect the above respiratory viruses.
Of seventy-five specimens, at least one virus was detected in 8 (10.7%) by DFT and in 69 (92%) by rtPCR. The most common viruses detected were RSV-A (5.4%), adenovirus (2.6%) PIV type1/3 (1.3%) by DFT and AdV (70.7%), RSV-A (42.6%), PIV1/3(14.6%), Flu A (8%), and PIV-2/4(1.3%) by rtPCR. Real-time PCR yielded more significant results than DFT in detection of respiratory viruses.
How to Cite:
Harshani, H.B.C., Ratnayake, N.R. and Jayamaha, C.J.S., 2022. Comparison of direct fluorescent test with real-time PCR to detect acute lower respiratory tract virus infections in children from May to July 2014, in a tertiary care hospital in Sri Lanka. Sri Lankan Journal of Infectious Diseases, 12(2), pp.E22 1–4. DOI: http://doi.org/10.4038/sljid.v12i2.8478
Published on
26 Jul 2022.
Peer Reviewed
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