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Comparison of direct fluorescent test with real-time PCR to detect acute lower respiratory tract virus infections in children from May to July 2014, in a tertiary care hospital in Sri Lanka

Authors:

H. B. C. Harshani ,

Medical Research Institute, Colombo, LK
About H. B. C.
National Influenza Centre, Department of Virology
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N. R. Ratnayake,

Lady Ridgeway Hospital, Colombo, LK
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C. J. S. Jayamaha

Medical Research Institute, Colombo, LK
About C. J. S.
National Influenza Centre, Department of Virology
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Abstract

In this study, direct fluorescent test (DFT) and real-time PCR (rtPCR) were compared for the detection of acute lower respiratory tract virus infections caused by respiratory syncytial virus (RSV) A and B, influenza (Flu) A and B, parainfluenza virus (PIV) 1, 2, 3 and 4 and adenovirus (AdV) in children admitted to the Lady Ridgeway Hospital, Colombo.

 

Nasopharyngeal aspirates/swabs were obtained from May to July, 2014. Cell pellets were subjected to DFT. Extracted viral RNA were subjected to four different real-time multiplex PCR assays to detect the above respiratory viruses.

 

Of seventy-five specimens, at least one virus was detected in 8 (10.7%) by DFT and in 69 (92%) by rtPCR. The most  common  viruses detected were RSV-A (5.4%), adenovirus (2.6%) PIV type1/3 (1.3%) by DFT and AdV (70.7%), RSV-A (42.6%), PIV1/3(14.6%), Flu A (8%), and PIV-2/4(1.3%) by rtPCR. Real-time PCR yielded more significant results than DFT in detection of respiratory viruses.
How to Cite: Harshani, H.B.C., Ratnayake, N.R. and Jayamaha, C.J.S., 2022. Comparison of direct fluorescent test with real-time PCR to detect acute lower respiratory tract virus infections in children from May to July 2014, in a tertiary care hospital in Sri Lanka. Sri Lankan Journal of Infectious Diseases, 12(2), pp.E22 1–4. DOI: http://doi.org/10.4038/sljid.v12i2.8478
Published on 26 Jul 2022.
Peer Reviewed

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